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KMID : 1037020130020020058
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Medical Lasers; Engineering, Basic Research, and Clinical Application
2013 Volume.2 No. 2 p.58 ~ p.63
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Usefulness and Limitations of Skin Explants to Assess Laser Treatment
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Cho Hong-Il
Won Chong-Hyun
Chang Sung-Eun
Lee Mi-Woo
Park Gyeong-Hun
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Abstract
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To overcome the ethical issues associated with performing clinical trials or obtaining patient’s skin tissues, there have been many attempts to develop appropriate models to study skin physiology, such as the use of skin explants to assess the effects of laser treatment. Skin explants are produced by processing skin tissue obtained from an excision. Contaminants and subcutaneous fat tissue are removed from the tissue, which is then cultured. Such processed tissue lacks blood circulation and nerve innervation, and is difficult to be preserved over a prolonged period of time. However, due to the similarities between skin explants and live skin tissues in terms of structure and physiological factors, such explants are thought to be useful as skin models to study the effects of laser treatment. Our previous studies showed that, following exposure to actual fractional laser, skin explants have very similar histological and molecular biological changes to those seen in the initial in vivo stages. However, with time, the differences between skin explants and live skin tissues become increasingly apparent. Therefore, skin explant models may be improved, such as by optimizing the culture conditions. In our present review, we examined the usefulness and limitations of skin explants to study the effects of laser therapy based on our own previous experience and the existing literature.
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KEYWORD
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Skin explant, Laser study, Fractional laser
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